SCRaMbLE of Heterozygous Diploids

NIAID Data Ecosystem2026-05-26 收录

合成生物学定向进化

酵母表型工程

数据链接：

https://www.ncbi.nlm.nih.gov/sra/SRP136404 数据链接链接失效反馈

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资源简介：

Here we use the Sc2.0 SCRaMbLE system to rapidly evolve heat-tolerant and caffeine-tolerant phenotypes in heterozygous diploid strains bearing one or more synthetic chromosomes.

创建时间：

2019-04-30

Engineered bacterial orthogonal DNA replication system for continuous evolution

合成生物学定向进化

DNA突变频率分析

High-throughput sequencing data of the mutation frequencies of two types of samples: samples at the beginning of the passage (S1-S3, to calculate sequencing errors) and error-prone DNAP samples at gen

NIAID Data Ecosystem70

SCRaMbLE of Heterozygous Diploids

合成生物学定向进化

耐受性表型筛选

Here we use the Sc2.0 SCRaMbLE system to rapidly evolve heat-tolerant and caffeine-tolerant phenotypes in heterozygous diploid strains bearing one or more synthetic chromosomes.

NIAID Data Ecosystem30

RNA sequencing of BHK-21 cells carrying alphaviral RNA replication system. RNA sequencing of BHK-21 cells carrying alphaviral RNA replication system

合成生物学定向进化

哺乳动物细胞转录组学

Directed evolution in mammalian cells can facilitate the engineering of mammalian-compatible biomolecules and can enable synthetic evolvability for mammalian cells. We engineered an orthogonal alphavi

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TALENs-Assisted Multiplex Editing for Accelerated Genome Evolution To Improve Yeast Phenotypes

酵母基因工程

合成生物学定向进化

Genome editing is an important tool for building novel genotypes with a desired phenotype. However, the fundamental challenge is to rapidly generate desired alterations on a genome-wide scale. Here, w

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Deep sequencing and fitness calculation for plasmids carrying the CREATE cassette from the enriched tolerant strains under different pressures by using Illumina protocol. Deep sequencing and fitness calculation for plasmids carrying the CREATE cassette from the enriched tolerant strains under different pressures by using Illumina protocol

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微生物耐受性

We report using global regulator libraries based on the CRISPR-enabled trackable genome engineering (CREATE) method to engineer tolerance against multiple inhibitors in Escherichia coli. Deep mutagene

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SCRaMbLE of Heterozygous Diploids

资源简介：

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