ExtData_Figure_7_qPCR
收藏DataCite Commons2025-04-01 更新2024-08-18 收录
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https://figshare.com/articles/dataset/ExtData_Figure_7_qPCR/22714579/1
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资源简介：
<em>(B) Testing of luciferase reporters with sequence insertions in the introns, as shown in diagram in A. HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with luciferase reporters containing the 99 bp insertions in </em>STOML2 or YKT6 <em>either in an exon or an intron, with or without PR8 PA-X. RNA was extracted and luciferase mRNA levels were quantified by qRT-PCR and normalized to 18S. Two sets of luciferase primers were used - either both annealing to exons or one to exon and one to intron, to measure levels of the processed mRNA and unspliced pre-mRNA, respectively.</em>
提供机构：
figshare
创建时间：
2023-05-01
