Table1_The Anti-Inflammatory and Anti-Pruritus Mechanisms of Huanglian Jiedu Decoction in the Treatment of Atopic Dermatitis.XLSX

Atopic dermatitis (AD) is a common chronic skin disease driven by a T-cell-mediated immune response, with inflammation and pruritus being its main clinical manifestations. Huanglian Jiedu decoction (HLJDT), which is an ancient Chinese medicine herbal formula derived from Wai-Tai-Mi-Yao, is a potentially effective treatment for AD. We aimed to clarify the anti-inflammatory and anti-pruritus mechanisms of HLJDT in AD treatment. We performed immunohistochemistry, Western blotting, reverse transcriptase-polymerase chain reaction, Luminex-based direct multiplex immunoassay, enzyme-linked immunosorbent assays, and flow cytometry to address the abovementioned aims. HLJDT significantly reduced clinical symptoms and ear swelling in AD-like mice by inhibiting the production of cytokines [histamine, interleukin (IL)-3, IL-4, IL-5, IL-13, IL-17A, IL-31, and IL-33], substance P (SP), transient receptor potential cation channel subfamily V member 1 (TRPV-1), and gastrin-releasing peptide (GRP). Additionally, HLJDT significantly suppressed the protein expression levels and positive cell percentage of CD28, CD80, CD86, CD207, CD326, MHCII, and OX40 in the lymphoid nodes. Moreover, HLJDT significantly suppressed mRNA and protein expression of tyrosine–protein kinase (JAK1), histamine H4 receptor, and IL-4Rα, as well as the protein expression of GRP, SP, and TRPV-1 in the root ganglion. Our findings indicate that HLJDT can treat AD by regulating the antigen presentation function of dendritic cells, weakening T-lymphocyte activation, and subsequently exerting anti-inflammatory and anti-pruritus effects.

特应性皮炎（AD）是一种由T细胞介导的免疫反应驱动的常见慢性皮肤病，其主要的临床表现为炎症和瘙痒。黄连解毒汤（HLJDT），源自《外台秘要》这一古老的中药方剂，对于AD的治疗具有潜在的有效性。本研究旨在阐明HLJDT在AD治疗中抗炎和止痒的机制。我们通过免疫组化、Western blotting、逆转录聚合酶链反应、基于Luminex的直接多重免疫分析、酶联免疫吸附测定和流式细胞术等手段，以实现上述目标。HLJDT通过抑制细胞因子[组胺、白介素（IL）-3、IL-4、IL-5、IL-13、IL-17A、IL-31和IL-33]、神经肽P（SP）、瞬时受体电位阳离子通道V家族成员1（TRPV-1）和胃泌素释放肽（GRP）的产生，显著减轻了AD样小鼠的临床症状和耳部肿胀。此外，HLJDT显著抑制了淋巴组织中CD28、CD80、CD86、CD207、CD326、MHCII和OX40蛋白的表达水平及阳性细胞百分比。同时，HLJDT显著下调了酪氨酸-蛋白激酶（JAK1）、组胺H4受体和IL-4Rα的mRNA和蛋白表达，以及根神经节中GRP、SP和TRPV-1的蛋白表达。我们的研究结果表明，HLJDT可以通过调节树突状细胞的抗原呈递功能、减弱T淋巴细胞活化，从而发挥抗炎和止痒的作用。