Data for: Plastic vasomotion entrainment in eLife

The presence of global synchronization of vasomotion induced by
oscillating visual stimuli was identified in the mouse brain. Endogenous
autofluorescence was used and the vessel “shadow” was quantified to
evaluate the magnitude of the frequency-locked vasomotion. This method
allows vasomotion to be easily quantified in non-transgenic wild-type mice
using either the wide-field macro-zoom microscopy or the deep-brain fiber
photometry methods. Vertical stripes horizontally oscillating at a low
temporal frequency (0.25 Hz) were presented to the awake mouse and
oscillatory vasomotion locked to the temporal frequency of the visual
stimulation was induced not only in the primary visual cortex but across a
wide surface area of the cortex and the cerebellum. The visually induced
vasomotion adapted to a wide range of stimulation parameters. Repeated
trials of the visual stimulus presentations resulted in the entrainment of
the amplitude of the vasomotion. Horizontally oscillating visual stimulus
is known to induce horizontal optokinetic response (HOKR). The amplitude
of the eye movement is known to increase with repeated training sessions
and the flocculus region of the cerebellum is known to be essential for
this learning to occur. Here, we show a strong correlation between the
average HOKR performance gain and the vasomotion entrainment magnitude in
the cerebellar flocculus. Therefore, the plasticity of vasomotion and
neuronal circuits appeared to occur in parallel. Efficient energy delivery
by the entrained vasomotion may contribute to meeting the energy demand
for increased coordinated neuronal activity and the subsequent neuronal
circuit reorganization.