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ena-DATASET-ErasmusMC-hema-05-05-2020-08:23:09:334-464 - samples

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https://www.omicsdi.org/dataset/ega/EGAD00001006106
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RNA was isolated using phenol-chloroform extraction followed by DNase digestion or using the Qiagen Allprep DNA/RNA kit and protocol (Qiagen, #80204). cDNA synthesis was done using the SuperScript II Reverse Transcriptase kit (Invitrogen). Quantitative real-time PCR was performed by using primers as described previously13,21 on the 7500 Fast Real-time PCR System (Applied Biosystems). Relative levels of gene expression were calculated using the ΔΔCt methodEGA dataset EGAD00001006106
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2020-07-16
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