Jennifer Bestman, Holly Cline (2011) CIL:36936, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is an image of a neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.

The cell was transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

此图像展示了一只白化非洲爪蟾(Xenopus laevis)神经管中，视束部位的神经元，处于47期发育阶段。该神经元细胞在图像采集前约24小时，通过体内电穿孔法转染了编码eYFP的质粒DNA。完整的活体蝌蚪在麻醉状态下，被置于载玻片下，并通过其透明的皮肤直接使用定制构建的双光子显微镜获取了此图像。