Raw data of the protection assay conducted on Spiroplasma-transinfected Drosophila melanogaster CS, with Leptopolina heterotoma.

Figshare2021-02-03 更新2026-04-08 收录

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https://figshare.com/articles/dataset/Raw_data_of_the_protection_assay_conducted_on_Spiroplasma-transinfected_Drosophila_melanogaster_CS_with_Leptopolina_heterotoma_/13686172/1

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Description of data: The dataset contains a record of the number of adult flies and wasps that emerged following parasitoid wasp attack. D. melanogaster naturally infected with Spiroplasma MSRO were used as a positive control. Data is in xlsl format. Description of methods for data collection: F2 generation D. melanogaster larvae were exposed to L. heterotoma wasps, following artificial infection with Spiroplasma, Hy1. Six treatments were established: transinfected larvae with and without wasps (S+ Lh+, S+ Lh-), uninfected controls with and without wasps (S- Lh+, S- Lh-) and a positive control naturally infected D. melanogaster with and without wasps (MSRO Lh+, MSRO Lh-). For each treatment 10 replicate vials were established, each containing 20 first and second instar Drosophila larvae. The wasps were permitted to attack larvae for 3 days at 25°C under a 12 h:12 h light:dark cycle. The number of fly pupae, eclosed adult flies and the number of eclosed wasps were counted for each vial. Column headings: Column A- wasp_presence: presence (Lh+) or absence of (Lh-) of L. heterotoma wasps Column B- strain: strain of D. melanogaster used. D. mel is the Canton S strain in which Spiroplasma was artificially injected into. MSRO is the D. melanogaster strain naturally infected with Spiroplasma and was used as a control. Column C- rep: replicate line of transinfected flies. Column D- infection_status: Spiroplasma infection status of the Drosophila larvae. Infection derived from artificial transinfecton (S+), Uninfected control (S-) and Drosophila naturally infected with Spiroplasma MSRO strain (MSRO). Column E- larvae: number of larvae placed onto the surface of Drosophila food. Column F- pupae: number of pupae that developed in each vial Column G- fly: number of adult flies that emerged from fly pupae Column H- no_fly: number of adult flies that did not emerge from fly pupae Column I- wasp: number of adult wasps that emerged from fly pupae Column J- no_wasp: number of adult wasps that did not emerge from fly pupae Column K- wasp_survival: proportion of adult wasps that emerged from fly pupae Column L- fly_survival: proportion of adult flies that emerged from fly pupae

创建时间：

2021-02-03