Primers used for site-specific mutagenesis.
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a Name of primer for each mutant based on numbering of mature FHbp ID 1 sequence, beginning with lipidated cysteine residue; F, forward; R, reverseb Sequence shown from 5’ to 3’; nucleotide changes relative to wild-type sequence in forward primer are underlinedc Annealing temperature used to amplify mutant FHbp genePrimers used for site-specific mutagenesis.
创建时间：
2015-12-03
