The dissociation constants (Kd) of AZ-ODC and AZ-AZI were derived from global fitting of the sedimentation velocity data to the model of A+B↔AB hetero-association in the SEDTHAT program.
SH3GL1 was fused to BioID2 biotinylase and expressed in Ramos Burkitt lymphoma cell line. Biotinylated proteins were immunoprecipitated after 14 hours. Eluted peptide extracts were resolved on an EASY