Exploring mechanisms of scar-free skin wound healing in adult zebrafish

We performed single-cell RNA sequencing to better characterize the dynamics and heterogeneity of involved cell types during different stages of zebrafish cutaneous wound healing. Overall design: Full-thickness wounds of adult zebrafish with a diameter of 2 mm were introduced with a clinical dermatology laser. For single cell RNA sequencing, single cell suspensions were prepared. For this task, unwounded skin tissue from three adult zebrafish was collected. In addition, the wound tissues for 2 dpw, 4 dpw and 6 dpw were collected using a biopsy punch with a diameter of 4 mm from three adult zebrafish each.