Medium composition for somatic embryogenesis of Medicago sp. plant and use thereof
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The present invention is a medicago genus containing 1-naphthaleneacetic acid (NAA) as auxin and Thidiazuron (TDZ) as cytokinin (Cytokinin) Medicago) It provides a medium composition for the development of somatic embryos of plants. The medium composition for somatic cell embryo development of a plant of the genus Medica according to the present invention contains a combination of new hormones as compared to a medium for generating somatic cell embryos of a conventional Medicago trankatula, and thereby, the somatic cell embryo generation efficiency is improved by about 10 times or more. Have. In addition, if the method for transforming a plant of the genus Medica according to the present invention is used, it is possible to stably transform the reference genotype A17 of Medicago truncula, which was very difficult to transform with the conventional method. Therefore, using the present invention, genome-based gene function studies and genetic manipulation of the reference genotype A17 of Medicago truncatula are possible.



