The first chromosomal-level genome assembly of an allotetraploid oyster

The allotetraploid oyster was artificially induced between the Portuguese oyster C. angulata and the Pacific oyster C. gigas. First, allotriploids were produced in 2015 by mating diploid C. angulata and autotetraploid C. gigas. Second, allotetraploids were produced in 2018 with the Guo and Allen method using eggs from the allotriploids and sperm from diploid C. angulata. Subsequently, allotetraploids were reproduced by 4n x 4n crosses for three generations. For this study, one allotetraploid oyster was sampled on 05/27/2024 from the F3 allotetraploids that were produced in 2022.