Transcriptome analysis of Indian mulberry leaf tissue

The mian goal of the study was to identify drought responsive genes of Indian mulberry (Morus alba L.) leaf tissue. Drought stress was imposed at whole plant leve; usig gravimetric approach. Leaf tissue was harvested 14 days post drought stress imposition and used for transcriptome analaysis. Two levels of drought stress (100% and 40% soil field capacity) was maintained by controlled irrigation. The leaf tissue was collected, total RNA was isolated converted to cDNA and used for analysis Overall design: 4 samples representing 2 treatments (fully irrigated and drought stress ie 100% and 40% field capacity) with two biological replicates each.