Table_1_Fusarioid community diversity associated with conifer seedlings in forest nurseries across the contiguous USA.xlsx

IntroductionFusarioid fungi that cause damping-off and root diseases can result in significant losses to conifer crops produced in forest nurseries across the USA. These nurseries are vital to reforestation and forest restoration efforts. Understanding the diversity of Fusarioid fungi associated with damping-off and root diseases of conifer seedlings can provide an approach for targeted management techniques to limit seedling losses and pathogen spread to novel landscapes.MethodsThis study identifies 26 Fusarium spp. (F. acuminatum, F. annulatum, F. avenaceum, F. brachygibbosum, F. clavus, F. commune, F. cugenangense, F. diversisporum, F. elaeagni, F. elaeidis, F. flocciferum, F. fredkrugeri, F. fujikuroi, F. grosmichelii, F. ipomoeae, F. lactis, F. languescens, F. luffae, F. odoratissimum, F. oxysporum, F. queenslandicum, F. redolens, F. torulosum, F. triseptatum, F. vanleeuwenii, & F. verticillioides), 15 potential species within Fusarium and Neocosmospora species complexes (two from F. fujikuroi species complex, nine from F. oxysporum species complex, three from F. tricinctum species complex, and one from Neocosmospora species complex), and four Neocosmospora spp. (N. falciforme, N. metavorans, N. pisi, & N. solani) and associated host information collected from conifer-producing nurseries across the contiguous USA.ResultsPhylogenetic analyses identified Fusarioid fungi haplotypes that were associated with 1) host specificity, 2) localization to geographic regions, or 3) generalists found on multiple hosts across diverse geographic regions.DiscussionThe haplotypes and novel species identified on conifer seedlings should be considered for further analysis to determine pathogenicity, pathogen spread, and assess management practices.

引言：引发苗腐病和根腐病的毛霉属真菌，对美国森林苗圃中生产的针叶作物造成了显著损失。这些苗圃对于造林和森林恢复工作至关重要。了解与针叶幼苗苗腐病和根腐病相关的毛霉属真菌的多样性，可以为制定针对性的管理技术提供途径，以限制幼苗损失和病原体向新景观的传播。
方法：本研究识别了来自美国大陆各针叶苗圃的26种毛霉属（F. acuminatum、F. annulatum、F. avenaceum、F. brachygibbosum、F. clavus、F. commune、F. cugenangense、F. diversisporum、F. elaeagni、F. elaeidis、F. flocciferum、F. fredkrugeri、F. fujikuroi、F. grosmichelii、F. ipomoeae、F. lactis、F. languescens、F. luffae、F. odoratissimum、F. oxysporum、F. queenslandicum、F. redolens、F. torulosum、F. triseptatum、F. vanleeuwenii、& F. verticillioides）物种，15种潜在物种（包括来自F. fujikuroi物种复合体的两种、来自F. oxysporum物种复合体的九种、来自F. tricinctum物种复合体的三种，以及来自Neocosmospora物种复合体的一种），以及四种Neocosmospora属（N. falciforme、N. metavorans、N. pisi、& N. solani）物种及其相关宿主信息。
结果：系统发育分析识别了与以下三种特征相关的毛霉属真菌单倍型：1）宿主特异性；2）地理区域的定位；3）在多个宿主和不同地理区域发现的泛化型。
讨论：在针叶幼苗上识别出的单倍型和新型物种，应考虑进行进一步分析，以确定致病性、病原体传播情况，并评估管理实践。