Regulation of HNF4α and its target genes in Zucker diabetic fatty (ZDF) rats.

Gene expression was measured by real-time qRT-PCR in 14 weeks and 9 months old ZDF rats and lean controls (n = 10 animals, respectively) and was determined relative to expression of cyclophilin, which served as a housekeeping gene. Gene expression in control rats was set to 100 and values for ZDF rats represent transcript abundance relative to control. Non-parametric Mann-Whitney-U-Test was used to compare ZDF and control groups. Results are considered significant at p[43].