Validation of Biomarker Expression in Intestinal Ischemia Reperfusion Injury: Insights from Sequencing Data and Experimental Analysis. Mus musculus

Intestinal ischemia-reperfusion injury (IIRI) is associated with significant clinical manifestations. Hence, it was vital to screen the important biomarkers for the diagnosis and treatment of IIRI. The transcript sequence data was used to screen out differentially expressed genes (DEGs) in IIRI. WGCNA was conducted to identify key module genes associated with IIRI compared to normal groups. A Venn Diagram was utilized to identify candidate genes. Further, a PPI network of candidate genes was established to demonstrate the interaction relationships. LASSO and SVM-RFE analyses were implemented to screen biomarkers of IRI. We finally conducted immune infiltration and functional enrichment analysis using biomarkers. Finally, the vital biomarkers in IIRI were verified by quantitative PCR and immunohistochemistry.