NMR data for RG-I isolated from Arabidopsis thaliana siliques

Pectin is generally divided into four distinct structural categories, namely homogalacturonan, xylogalacturonan, rhamnogalacturonan I (RG-I), and rhamnogalacturonan II. While much of the structural diversity of homogalacturonan, xylogalacturonan, and rhamnogalacturonan II has been elucidated, the structural features of RG-I are less well understood. In this work, we employed multiple complementary analytical techniques to present a detailed structural analysis of RG-I in the model system Arabidopsis thaliana. Starting with highly purified RG-I from different Arabidopsis tissues, we employed comparative linkage and NMR analysis along with mass spectrometry analysis of enzymatically digested RG-I oligosaccharides. Besides the presence of the canonical 5-arabinan, 4-galactan, 6-galactan, and arabino-galactan RG-I side chains of varying lengths, we show that a large portion of the 6-galactan is terminated by either 4-O-methyl β-GlcA residues or, to a smaller degree, β-GlcA that lacks the Me..., , , # NMR data for RG-I isolated from Arabidopsis thaliana siliques

[https://doi.org/10.5061/dryad.8gtht7707](https://doi.org/10.5061/dryad.8gtht7707)

## Description of the data and file structure

This dataset contains NMR data acquired for rhamnogalacturonan I (RG-I) isolated from *A. thaliana* silique tissues. It contains raw and processed NMR data in the Bruker format that were acquired on rhamnogalacturonan I (RG-I) isolated from Arabidopsis thaliana silique tissue.

### Files and variables

#### File: deposition.zip

**Description:**

The NMR data for the sample were collected at two different pD values: 4.7 and 1.7, and are saved in the respective directories \"At_silique_RG-I_pD4.7\" and \"At_silique_RG-I_pD1.7\".

The NMR data are in the standard Bruker format, and the directories contain the raw as well as the processed data for each spectrum as they were used for the analysis.

Each of the two data directories contains a notes.txt file that lists the NMR experiments included in the...,