Ovarian Stroma RNA-seq of Progesterone receptor null vs WT mice at 8hrs post hCG

Progesterone receptor-dependent expression of the ovairan stroma is investigated to determie PGR control of stromal functions at ovulation. Ovaries were collected from 21-25day old PGR(-/-) and PGR(+/+) littermates at 8hrs post-hCG. N=3-4 per genotype with each experimental replicate consisting of tissue pooled from 3 mice. The Stromal tissue of the ovary (GC-depleted ovary) was used for mRNA-seq Illumina Truuseg Strandard mRNA kit, Illumina Novaseq 600 SP. Paired end, 100bp, depth of 54-72M reads. Qualisty control checked using FastQC. Salmon lignment to GRCm39 M26 mouse transcriptome. DESeq2 used to asses differentially expressed genes.