Target enrichment and transcriptome sequencing of multiple species of Cypripedium

We used the orchid-specific bait set "Orchidaceae963" to reconstruct the phylogeny of Cypripedium covering all section sections. Subsequently, we investigated gene tree discordance, estimated divergence times and ancestral ranges, searched for anomaly zones, polytomies, and diversification rate shifts, and identified gene duplication and hybridization events.