Supplemental Material for Pujari and Cullen, 2024

Signaling pathways control the response to stimuli. In yeast, a signaling (MAPK) pathway controls a fungal behavioral response called filamentous growth. A genetic screen was performed to identify spontaneous mutants that show hyperactivity of a MAPK pathway-dependent reporter. Select mutants were analyzed by whole-genome sequencing. New alleles in known regulatory proteins were identified, including an inhibitory domain in the C-terminus of the transcription factor Ste12p. Our results highlight the combinatorial effects of multiple positive and negative regulatory inputs in regulating a MAPK-dependent differentiation response.