Meta-analysis of Single-cell RNA-seq Data Reveals the Formation of Tertiary Lymphoid Organ and Mechanism Heterogeneity in Vascular Disease

The role of different cells in intimal hyperplasia remains unintelligible. To characterize the cells in intimal hyperplasia, we performed single-cell RNA sequencing of whole remodeled vessel cells from C57BL/6J mice with a murine model of intimal hyperplasia at an early (IH 2w) or late stage (IH 4w). The single-cell RNA-seq analyses revealed the heterogeneity of cells in vascular remodeling. Single-cell RNA sequencing of whole vessel cells from the remodeled carotid artery of a murine model of intimal hyperplasia at 2-week time point (n=18, eight weeks-age). Single-cell RNA sequencing of whole vessel cells from the remodeled carotid artery of a murine model of intimal hyperplasia at 4-week time point (n=18, eight weeks-age).