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Mapping of nascent RNA in MLL2 F/F and FC/FC MEF cells. Mus musculus

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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA296117
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Global Run-On has been performed in MLL2 F/F and FC/FC MEF cells. The aim of the experiment is to test how the lack of MLL2 affects nascent RNA transcription. Overall design: Four samples were analyzed - 2 independent replicates. The experiments were performed using immortalized mouse embryonic fibroblasts (MEF) in which both MLL2 alleles were targeted by the loxp system (F/F cells). Tamoxifen treatment of the F/F cells for 24 hours results in the excision of both MLL2 alleles (FC/FC cells).
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2015-09-17
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