Figure 5

Figure 5 | Activation of hM3Dq signaling in astrocytes does not alter baseline behavior. a, Experimental approach and timeline of clozapine-N-oxide (CNO) treatment and behavioral assessments. All mice received CNO (1mg/kg i.p.) 30 minutes before each behavioral task. b, Total distance traveled of an open field (n=7 male astro-hM3Dq, 11 female astro-hM3Dq, 13 male control, and 14 female control). c, Time spent in the centre of the locomotor apparatus (n=7 male astro-hM3Dq, 11 female astro-hM3Dq, 13 male control, and 14 female control). d, Time spent on the light side of a light/dark box (n=7 male astro-hM3Dq, 11 female astro-hM3Dq, 13 male control, and 14 female control). e, Time spent in the open arm of an elevated plus maze (n=7 male astro-hM3Dq, 11 female astro-hM3Dq, 13 male control, and 14 female control). f, Percentage of spontaneous alternations in Y maze (n=7 male astro-hM3Dq, 11 female astro-hM3Dq, 13 male control, and 14 female control). g, Time spent interacting with a juvenile mouse in a three-chamber social preference task (sociability) (n=7 male astro-hM3Dq, 11 female astro-hM3Dq, 13 male control, and 12 female control). h, Time spent interacting with a novel juvenile mouse in a three-chamber social novelty task (social recognition) (n=7 male astro-hM3Dq, 11 female astro-hM3Dq, 13 male control, and 12 female control). Each symbol on the graph represents an individual animal (blue=males, pink=females). Data are presented as meanSEM. Unpaired, two-tailed Student t-test. ns>0.05.