Valproic acid-induced changes of 4D nuclear morphology in astrocyte cells

Imaging dataset accompanying the paper: Kalinin AA, Hou X, Ade AS, Fon GV, Meixner W Higgins GA, Zheng G, Sexton JZ, Wan X, Athey BD, O’Meara MJ, Dinov ID. 2018. Valproic acid-induced changes of 4D nuclear morphology in astrocyte cells. Molecular Biology of the Cell, 2(18), 1624-1633. doi: 10.1091/mbc.E20-08-0502 Dataset description: The dataset consists of 3D fluorescence microscopy images of primary human astrocytes (hippocampal). The dataset contains astrocytes in two different phenotypic states: normal human astrocytes cutured in DMSO (NHA) and astrocytes treated with 1.5 mM valproic acid (VPA), imaged at three timepoints (days 3, 5, and 7). Astrocyte nuclei were labeled with DAPI (4',6-diamidino-2-phenylindole) and imaged using Zeiss LSM 710 laser scanning confocal microscope with a 63x PLAN/Apochromat 1.4 NA DIC objective. Original 3D volumes were then cut into Zx1024x1024 sub-volumes and deconvolved using the Lucy-Richardson deconvolution algorithm (see paper for details). The dataset also includes segmented nuclei: 512 NHA masks and 320 VPA masks. NHA: Day 3: images and masks (7.6 GB total) Day 5: images and masks (8.5 GB total) Day 7: images and masks (4.5 GB total) VPA: Day 3: images and masks (4.8 GB total) Day 5: images and masks (3.0 GB total) Day 7: images and masks (6.9 GB total)