Cerebellar Purkinje cell whole cell voltage clamp mIPSC recordings in Dag1 conditional mutant mice

Here we studied the role of Dystroglycan in the function of inhibitory cerebellar synapses from MLIs onto Purkinje cells. In all cases, we recorded mIPSCs from Purkinje cells held at -70mV in whole cell voltage clamp. The bath contained NBQX and TTX. Recordings were performed using Igor Pro 8 (Wavemetrics) and files are in the proprietary .pxp format. Genotype information for each recording can be found in the phys_metadata.csv file in this dataset. Igor code for mIPSC analysis can be found at https://github.com/jnjahncke/mini_analysis (for publication, a cutoff of -12000 was used).
We performed experiments under 5 different genetic conditions:
Calb1Cre;Dag1cKO - Dag1 deletion starting ~E14 (recordings performed at P30)Calb1Cre;Pomt2cKO - Loss of Dag1 glycosylation starting ~E14 (recordings performed at P30)Calb1Cre;Dag1cΔICD - Loss of Dag1 intracellular domain starting ~E14 (recordings performed at P30)Pcp2Cre;Dag1cKO - Dag1 deletion starting ~P14 (recordings performed at P30)Pcp2Cre;Dag1cKO - Dag1 deletion starting ~P14 (recordings performed at P60)