scRNA-seq data from: DJ-1 inhibition reshapes tumor microenvironment and potentiates immune checkpoint inhibitors

This dataset contains raw single-cell RNA sequencing data generated from tumor-infiltrating CD45⁺ immune cells in murine cancer models to investigate the role of DJ-1 (PARK7) in modulating anti-tumor immunity. In the associated study, genetic ablation of DJ-1 enhanced the efficacy of immune checkpoint blockade by indirectly activating T cells through macrophage reprogramming. Loss of DJ-1 increased reactive oxygen species (ROS) levels in macrophages and promoting differentiation into Cxcl9⁺ immune-stimulatory phenotypes while reducing Spp1⁺ immune-suppressive subsets.
Here, we deposited additional raw sequencing reads in FASTQ format from sorted CD45⁺ cells (DJ-1 knockout and wild-type conditions).  A detailed description of experimental procedures and analysis workflows can be found in the associated publication.
, , # scRNA-seq data from: DJ-1 inhibition reshapes tumor microenvironment and potentiates immune checkpoint inhibitors

Dataset DOI: [10.5061/dryad.1zcrjdg6c](https://doi.org/10.5061/dryad.1zcrjdg6c)

## Description of the data and file structure

This dataset supplements the manuscript titled “DJ-1 Inhibition Reshapes Tumor Microenvironment and Potentiates Immune Checkpoint Inhibitors”. We performed single‑cell RNA sequencing of tumor‑infiltrating CD45⁺ immune cells to characterize transcriptional changes in T cell and myeloid populations following DJ‑1 knockout. All raw sequencing reads are deposited in FASTQ format. The *KO-antibody-T.fq.gz* and *WT-antibody-T.fq.gz* datasets correspond to the T cell–related TRC analyses in Figure 3, whereas the *KO1.fq.gz*, *KO2.fq.gz*, *WT1.fq.gz*, and *WT2.fq.gz* datasets correspond to the myeloid cell analyses in Figures 4 and 5. Detailed experimental and analytical methods are provided in the associated manuscript.

### Files and variables

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