Modulation of multistep cytokine induction in the activated microglia cell line MG5

Ocular cytokine network plays pivotal role for initiation and progression of retinal degeneration, and microglia participate ocular inflammation. LPS activates MG5, mouse microglial cell line, and protein synthesis inhibitor CHX modulates cytokine expression pattern. To reveal comprehensive transition of molecular events, we performed proteome analysis using MG5 cells, and found that various mRNA modifying molecules are induced after LPS stimulation in addition to the inflammatory related molecules. The expression of mRNA modifying molecules are suppressed significantly by CHX, suggesting that transient induction of the molecules plays roles for determine the phenotype of inflammation in the microglia.