A fission-yeast gene encoding a protein with features of protein-tyrosine-phosphatases.
收藏PubMed Central1991-04-15 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC51466/
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资源简介：
Degenerate oligonucleotide probes encoding sequences conserved among mammalian protein-tyrosine-phosphatases (PTPases) were used to amplify DNA fragments from a Schizosaccharomyces pombe cDNA library by polymerase chain reaction (PCR) methods. A cloned PCR product predicted peptide sequences similar to those found in PTPases but not identical to any published sequences. A S. pombe gene, designated pyp1+, was identified in a cDNA library with this PCR probe, cloned, and sequenced. The sequence of the gene predicted a 550-amino acid protein with Mr 61,586, which includes amino acid sequences that are highly conserved in mammalian PTPases. Disruption of the pyp1+ gene resulted in viable cells. Overexpression of the pyp1+ gene in S. pombe permitted detection of a protein of apparent Mr 63,000. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1991-04-15
