Gene expression and radiation response of two different types of neuroblastoma cell lines. Gene expression and radiation response of two different types of neuroblastoma cell lines

Clonogenic assays were used to establish radiation responses for SK-N-AS, S-type and SK-N-DZ, N-type cell lines; cells were then irradiated at doses that cause 90% cell killing based on clonogenic assay and their RNA was isolated one hour post-irradiation. In addition, cells were transfected with pre-miRNA constructs that led to overexpression of micro-RNAs hsa-miR-34a and hsa-miR-1228. Statistically significant differences were detected for expression of several thousands of genes when the two cell lines were compared with each other. In comparison, radiation led only to minor, less than two-fold gene expression differences. Overexpression of micro-RNAs in either cell line did not change this outcome. Overall design: Neuroblastoma cell line SK-N-AS was irradiated with 6Gy dose of X-rays 20-24h after transfection, cell line SK-N-DZ was processed the same way but the radiation dose was 4Gy. Gene expression at 1h after radiation was evaluated.