The role of CHD7 in human central nervous system development (ChIP-Seq)

CHARGE syndrome is a congenital disorder caused by mutations in Chromodomain Helicase DNA-binding domain 7 (CHD7) gene. We generated a cell line carrying HA-tag knock-in into the human CHD7 locus, mediated by CRISPR-Cas9 system. We then performed ChIP against HA antibody using the validated knock-in cells and subsequent high-throughput sequencing analysis. Overall design: Examination of histone modification and CHD7 binding sites in WT, CHD7-KD and CHARGE patient cells. CHARGE patient raw data is not available in GEO due to patient privacy restrictions. The raw data is available via controlled access through JGA (Japanese Genotype-Phenotype Archive) by accession number JGAS00000000131.