Translational activities of 3′NTR mutant replicons in cB76.1/Huh7 cells

aReplicon RNAs containing 3′NTR mutations or deletions correspond to those depicted in Fig. 5A, but encode firefly luciferase (F-Luc) in place of neomycine phosphotransferase. Luc-RepDΔIII and LucD-GAA control RNAs are described in the legend of Fig. 2.bF-Luc RLU activities were determined 4 hours after transfection of equal amounts (5 µg) of replicon RNAs into cB76.1/Huh7 cells, and expressed relatively to Luc-RepD set at 100% after normalization with respect to total protein quantities assayed (mean±SD from two independent transfections, each monitored in duplicate wells).