Summary of free running activity rhythms.

Related to Figs 2, 3, and7and S1–S4. Activity analysis of the above genotypes at 25°C, 28°C, or 18°C. Light conditions for each experiment was 12:12 LD for 5 days followed by DD for at least 8 days. Depending on the experiment, flies were raised at 25°C, 28°C, or 18°C. Flies raised a 25°C were kept at that temperature throughout the behavior experiment. Flies raised at 18°C were transferred to 28°C upon eclosion and behavior experiments were conducted at 28°C. Flies raised at 28°C were either kept at 28°C for behavior experiments (constitutive knockdown) or transferred to 18°C upon eclosion (development-specific knockdown). ClockLab’s χ-square periodogram analysis was used to analyze rhythmicity, rhythmic power, and free-running period for each above genotypes. The % rhythmicity along with the number of rhythmic flies (nR), the period in hours with the SEM, and the rhythmic power with the SEM are indicated. Arrhythmic flies were not included in the analysis of period or power.