High-frequency (2 kHz) sine wave stimulation of individual hippocampal neurons: the effect of electric field

2 kHz stimulation of hippocampal neurons under different amplitudes (measured as one polarity, V; peak-to-peak would be two-fold larger). Sine wave amplitude was gradually increased during the initial 0.25 s, then stayed constant at the voltage indicated in session descriptions. Sine wave stimulation begins at 25 ms and lasts 2 s. For the employed electrode configuration, 1V translates into 36 V/cm. Neuron action potentials were visualized using the voltage-sensitive fluorescent dye, FluoVolt. The study was supported in part by NIH 15R21EY034803.