Fe recovery of serum samples after SEC-ICP-AES analysis.

Using the SEC-ICP-AES area counts for Fe that were obtained for the analysis of a rabbit plasma stock for which the total Fe concentration was known allowed us to calculate how many μg of Fe injected corresponded to what total Fe area count. This correlation was then used to calculate the Fe recovery in % by using the total Fe of each serum sample (determined by ICP-MS) and the Fe area counts obtained after SEC-ICP-AES analysis.