m6A-seq and RNA-seq in Arabidopsis dcl1 mutant

N6-methyladenosine (m6A) modification is essential for plant growth and development. Recently, a possible involvement of miRNAs in m6A modification is reported in animal. To understand a potential involvement of miRNAs in sequence-specific deposition of m6A marks in plants, in this study, we performed m6A-seq and RNA-seq for a mutant of dicer-like 1 (dcl1), a key component in miRNA biogenesis in Arabidopsis. And the difference of m6A modification was compared between Col-0 mutant and dcl1 mutant. Overall design: The Arabidopsis seedling of Col-0 and dcl1 mutant seedlings were collected at 3-week-old growth stage. And the RNA was extracted and subjected to m6A and RNA sequencing with three biological replicates.