14-day AN11251 treatment reduces the Wolbachia burden by > 99% in all adult worms analyzed.

Shown are the number of female worms with less or more than 99% of Wolbachia reduction as well as the frequency of female worms, which presented a Wolbachia reduction greater than 99%. Female adult worms were isolated from wild-type BALB/c mice that had been infected for 35 days with L. sigmodontis and treated with different concentrations of AN11251 (50, 100, 200, 300, and 400 mg/kg), rifampicin (10 and 35 mg/kg) or doxycycline (40 and 100 mg/kg) for 7, 10 and 14 days or the vehicle control. The drug was given via the oral route as a twice-daily dosage (BID) or as a single dose per day (QD). AN11251: N = 5 per group, rifampicin and doxycycline: N = 4–5 animals per group (4 animals for 7 dpi doxycycline 40 mg/kg BID).