QPCTL deletion results in a rewiring of the tumor microenvironment [bulk RNA-seq]

bulk RNA sequencing performed on the FACS sorted CD45-negative cells from QPCTL-proficient and -deficient TMEs; and, base-line comparison of lymphoid organs from QPCTL-KO and QPCTL-WT animals The effects of QPCTL-deficiency were assessed in this study. To this end, a QPCTL-KO mouse strain was used. Mice homozygous for the QPCTL-KO allele or wild-type littermates were innoculated with either QPCTL-KO or QPCTL B16F10 tumor cells, respectively. After 14 days tumors were harvested, single cell suspensions were made and live CD45-negative cells were isolated by FACS, after which cells were processed for RNA sequencing. For baseline samples, whole organs were harvested from mice, snapfrozen and used for mRNA isolations.