Subcellular RNA localization during Drosophila embryonic neurogenesis [fractionation-Seq]
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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资源简介：
We isolated whole embryo, nuclear, and cytoplasmic lysates from Drosophila embryos during early and late embryogenesisIn Drosophila melanogaster, cell-type specification during early nervous system development requires precise regulation of gene expression in time and space. To investigate the gene regulation patterns in early neuronal development, we developed a method 'DIV-SortSeq' to enrich for specific neuroglial cell types for RNA sequencing: from early columnar subdivision and specification, through neuroglial differentiation. DIV-SortSeq recapitulates many of the known protein-coding transcriptome dynamics, as well as novel transcriptional regulatory patterns. We also performed nuclear-cytoplasmic fractionation of whole embryos - Fractionation-Seq - to assess temporal changes in subcellular localization of transcripts during embryogenesis. We present these data as a resource to permit further in-depth investigation into the functional roles of the coding and noncoding transcriptome during early Drosophila neurogenesis.
提供机构：
Max Delbrueck Center for Molecular Medicine
创建时间：
2022-02-20
