Replication Data for: glutathione of "Replicative Senescence in Mesenchymal Stem Cells: An In Vitro Study on Mitochondrial Dynamics and Metabolic Alterations"

The ratio of reduced (GSH)/oxidized (GSSG) glutathione was evaluated using the luminescence-based GSH/GSSG-Glo Assay (Promega, Madison, WI, USA). bMSCs seeded on a 96-well white plate (Costar, Sigma-Aldrich) (6 × 103 cells/well) were incubated with Total Glutathione Lysis Reagent or Oxidized Glutathione Lysis Reagent and shaken at room temperature for 5 min. Then, Luciferin Generation Reagent was added to each well and the cells were incubated at room temperature for 30 min. Luciferin Detection Reagent was then added to each well. After 15 min, luminescence was acquired using Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific).