Single cell RNA-seq of single cell suspension from the HCC region of WT and FABP5 Hepatocyte KO mice fed CD-HFD for 15 month

After feeding CD-HFD diet for 15 month, tumors from WT and FABP5 hepatocyte KO mice were collected and digested with collagenase to generate single cell suspension before proceeding with 10X Single Cell RNA-Sequencing. Overall design: The tumor region of WT and FABP5 Alb-CRE-ER mice were dissected after 15 month of CD-HFD. Single cell suspension was prepared and cells were submitted for sc-RNAseq