Data for: Techno-economic analysis of a novel laccase production process utilizing perennial biomass and the aqueous phase of bio-oil, Iowa, USA 2023-2025

This dataset contains the experimental design, measurements, and derived variables used to parameterize a techno‑economic model of laccase production via two‑stage solid‑state fermentation of prairie biomass with bio‑oil aqueous phase induction. It includes nutrient screening data for Pleurotus ostreatus growth on prairie biomass with alternative nitrogen sources and a corn‑steep solids dose series; factorial/response‑surface experiments varying substrate bed depth, substrate‑to‑inoculum (S:I) ratio, and pre‑induction growth time; and time‑resolved induction measurements. For each run and replicate, the data record the full set of spectrophotometric absorbances at 0–210 s, fitted slopes and r-square values, dilution and volume factors, and laccase activities normalized per mL and per gram of biomass, alongside the exact culture timings and environmental conditions used in the ABTS assay at 420 nm. Results tables provide the fitted central‑composite design model terms (coefficients, F‑statistics, and p‑values) used directly as inputs to the minimum laccase selling price (MLSP) calculations, together with the underlying per‑condition raw results.