Dose dependent effect of primaquine on the development of 3 day hepatocyte stage parasites.
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25,000 fresh Pv spz were added to wells containing 20,000 hepatoma cells and were cultured for 3 days with medium alone or in the presence of different concentrations of primaquine (PQ). The numbers of early liver stage trophozoites (3 day assay) were counted by immunoflourescence microscopy after staining with the anti-PvCSP mAb (NVS3) as described in Materials and Methods.
创建时间:
2015-12-02



