Transcriptome analysis of nascent hematopoietic cells

During ontogeny, hematopoietic stem cells (HSCs) and erythroid-myeloid progenitors (EMPs) are generated from distinct hemogenic endothelium. Nascent HSCs and EMPs are distinguished by the expression of Hlf. To obtain mechanistic insight into HSC specification, we compared the gene expression profiles between Hlf+ and Hlf- nascent hematopoietic cells. Overall design: Hlf+ and Hlf- nascent hematopoietic cells (c-Kit+CD45-) were isolated from embryonic day (E)10.5 caudal half and E9.5 yolk sac, respectively, and were subjected to RNA-seq analysis.