Data and R code for: “eDNA Metabarcoding Highlights Environmental Influence Over Biotic Interactions in Amphibian and Fungal Communities of Hampstead Heath Ponds”

This dataset contains environmental DNA (eDNA) metabarcoding data and statistical analysis code for the study “eDNA Metabarcoding Highlights Environmental Influence Over Biotic Interactions in Amphibian and Fungal Communities of Hampstead Heath Ponds.” The study assessed amphibian and fungal (Saprolegniales) communities across urban ponds at Hampstead Heath (London, UK). Two genetic markers were used: COI and 18S rRNA. The repository includes relative abundance matrices for all detected taxa and Saprolegniales-specific abundance data. It also provides alpha diversity indices (Shannon and Simpson), Bray–Curtis beta diversity matrices, and combined amphibian-fungal-abiotic datasets. An R script contains all statistical analyses and figure generation procedures. Statistical analyses were conducted in R v4.2.2, including diversity calculations, community dissimilarity analyses, ordination (NMDS/CCA), PERMANOVA, regression models, and correlation analyses.