Sequencing and assembly of saccharinae chloroplasts based on 12 long-range PCR primers. Primer-based Chloroplast Sequencing and Assembly

Representative chloroplast genomes from the saccharinae were amplified using 12 novel long-range PCR primers. The amplicons were pooled and sequenced with Illumina technology. The reads were cleaned, trimmed, normalized for coverage and then assembled using SPAdes before annotation