mRNA Profiling of Murine 4T1 Breast Cancer Cells with Asns knocked down. mRNA Profiling of Murine 4T1 Breast Cancer Cells with Asns knocked down

Murine 4T1-T Breast cancer cells were infected with retroviral vectors harboring shRNAs for murine or a Renilla, as negative control. After selection cells were harvested and processed with the NuGen Ovation RNAseq system V2. Libraries were sequenced on an Illumina platform and mapped using Bowtie2 to the mm10 genome prior to transcript quantification using HT-Count. Overall design: 4T1 Breast Cancer Cells with Asns knocked down