Proteins detected in SB623-derived ECM using In-Gel Digest and nLC-MS/MS or SAISD and nLC-MS/MS.

SDS/urea-soluble ECM from SB623 was precipitated and proteins separated using SDS-PAGE. Eight of the most prominent high molecular weight gel bands were excised. ECM protein was destained, reduced, alkylated, trypsinized, extracted and individually analyzed with nLC-MS/MS. For SAISD, SB623-derived ECM protein was resuspended in 0.1% w/v ammonium bicarbonate and Rapigest surfactant powder. The sample was then reduced, alkylated, trypsinized, and analyzed using nLC-MS/MS. Proteins identified had at least two unique peptides. Note, in-gel and SAISD ECM samples analyzed using nLC-MS/MS were from different donors.