Knockout of ER membrane protein complex subunits

The ER-membrane protein complex (EMC) functions as a transmembrane domain insertase with a broad client range, among which are proteins critical for maintaining cholesterol homeostasis. RNAseq was performed to determine whether the EMC-deficient cell lines elicited a transcriptional response to restore cholesterol homeostasis through the SREBP2 transcription factor. These data provide insight into the transcriptional programs that are activated when the insertase activity provided by the EMC is absent, from which key client proteins may be determined.