Immediate response of AOB to short-term fertilization and moisture changes

Samples were taken from Changsha, Hunan, with a total of 10 treatments, three parallel treatments per treatment. As for Q7-Q36, the processing includes: CK, NF; WFPS is 25%, 50%, 75%, 100%, and 125%.PCR products were pooled in equimolar proportions using PCR-purified AxyPrep DNA-purified amplicons on the Illumina Miseq platform (Illumina, San Diego, USA) for paired sequencing at Shanghai Myriad Biomedical Technology Co. Raw FASTQ files were demultiplexed and quality filtered using QIIME v1.8.0. All samples were normalised to a similar sequencing depth using MOTHUR. Operational clustering of taxonomic units (OTUs) was performed using UPARSE v7.1, based on a cut-off of 97% similarity, and chimeric sequences were identified and removed using UCHII. Identification and deletion were performed using UCHIIME. Representative sequences for each OUT were analysed using NCBI for each OUT and annotated according to the closest related sequence to the species-specific information. Representative sequences for each OUT were analysed using a 70% confidence threshold and annotated according to the closest related sequence with species-specific information