An inflamed human alveolar model for testing the efficiency of anti-inflammatory drugs in vitro

The data set accompanies the study where we developed an inflamed human alveolar epithelium model and to test the resolution lipopolysaccharide (LPS)-induced inflammation in vitro with a corticosteroid, methylprednisolone (MP). A specific focus of the study was in macrophage phenotype shifts in response to these stimuli.

The data set includes:

- Pro-inflammatory marker (interleukin (IL)-8, tumor necrosis factor α (TNFα), IL1β) secretion data, analysed via ELISA,  and cell viability (analysed via lactate dehydrogenase assay) of both monocultures (human monocyte-derived macrophages) and of the multicellular human alveolar model, composed of macrophages, dendritic cells, and epithelial cells. 

- Barrier permeability data of the multicellular model, assessed via labeled-dextran permeability assay. 

All the above-stated data is joined in the file: DraslerB_Frontiers 2020_Inflammatory model. Sample codes are explained int he first tabs. 

- Pro-inflammatory marker gene expression data of the multicellular model, assessed via real time RT-qPCR. The data prepared for analysis and analysed is joined to the above-mentioned folder, whereas the direct PCR runs are joined in the file: DraslerB_Frontiers 2020_Inflammatory model_PCR runs.  

- Confocal laser scanning microscopy raw files (lsm) of the multicellular model can be opened with an open source software Fiji, based on ImageJ.